Title

The effect of the fullerene nanoparticle on mitochondrial respiration

Presenter Information

Zachary Miller

Document Type

Oral Presentation

Location

SURC Ballroom C/D

Start Date

16-5-2013

End Date

16-5-2013

Abstract

C60, or fullerenes, are a controversial nanoparticle because of their unknown effects to biological systems. With increased research into many different industrial and medical applications for C60, such as commercial lubricants and medical delivery devices, scientists are concerned of the potential adverse effects these nanoparticles could have on human health. We investigated the potential adverse effects of C60 on respiration of bovine heart mitochondria by monitoring oxygen consumption in the presence of NADH. C60 was solubilized in 7.5 percent Bovine Serum Albumin to keep the nanoparticles from clumping. C60 was used in concentrations ranging from 2 to 35 parts per million and incubated with bovine heart mitochondria for up to 10 minutes before measuring oxygen consumption. The rate of oxygen consumption was collected using a Clark-electrode and the data were analyzed using MS Excel. Preliminary results show that oxygen consumption from bovine heart mitochondria in the presence of NADH (Complexes I, III and IV of the electron transport chain) was affected by different concentrations of C60 or time of incubation with C60.

Poster Number

40

Faculty Mentor(s)

Carin Thomas

Additional Mentoring Department

Chemistry

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May 16th, 8:20 AM May 16th, 10:50 AM

The effect of the fullerene nanoparticle on mitochondrial respiration

SURC Ballroom C/D

C60, or fullerenes, are a controversial nanoparticle because of their unknown effects to biological systems. With increased research into many different industrial and medical applications for C60, such as commercial lubricants and medical delivery devices, scientists are concerned of the potential adverse effects these nanoparticles could have on human health. We investigated the potential adverse effects of C60 on respiration of bovine heart mitochondria by monitoring oxygen consumption in the presence of NADH. C60 was solubilized in 7.5 percent Bovine Serum Albumin to keep the nanoparticles from clumping. C60 was used in concentrations ranging from 2 to 35 parts per million and incubated with bovine heart mitochondria for up to 10 minutes before measuring oxygen consumption. The rate of oxygen consumption was collected using a Clark-electrode and the data were analyzed using MS Excel. Preliminary results show that oxygen consumption from bovine heart mitochondria in the presence of NADH (Complexes I, III and IV of the electron transport chain) was affected by different concentrations of C60 or time of incubation with C60.