The Effects of 2-deoxy-D-glucose and Different E. coli Diets on C. elegans Mitochondrial Metabolism
Document Type
Oral Presentation
Campus where you would like to present
SURC Ballroom C/D
Start Date
16-5-2013
End Date
16-5-2013
Abstract
We have investigated the effects of different bacterial diets on C. elegans as a model for metabolic studies in obesity and diabetes research. Our goal was to understand C. elegans’ base line metabolism by measuring ATP content under different dietary conditions. This study examined three strains of E. coli that can be used as food for C. elegans. The E. coli strains OP50 and HB101 are used as regular diets and HT115, which is a derivative of HB101. It has previously been shown that OP50 has higher fatty acid composition and HB101 has higher carbohydrate composition. The ATP content in OP50 fed worms was significantly lower than the HB101 and HT115 fed worms in both wild-type and nnt-1 strains of C. elegans. The nnt-1 strain is deficient in a mitochondrial protein, nicotinamide nucleotide transhydrogenase (NNT-1), associated with type-2 diabetes. In addition to these baseline studies, 2-deoxy-D-glucose was used as an inhibitor of glycolysis as a method for forcing fat metabolism in C. elegans. This dietary model could then be used to investigate the effects of increased fat metabolism on mitochondrial function in C. elegans. The baseline results were reversed in the 2-deoxy-D-glucose experiments suggesting that the worms were relying on fat metabolism. Future studies are planned to measure mitochondrial membrane potential, hydrogen peroxide production, and respiration to further understand the effects of increased fat metabolism on the mitochondria.
Recommended Citation
Messerman, Hayden, "The Effects of 2-deoxy-D-glucose and Different E. coli Diets on C. elegans Mitochondrial Metabolism" (2013). Symposium Of University Research and Creative Expression (SOURCE). 126.
https://digitalcommons.cwu.edu/source/2013/posters/126
Poster Number
37
Additional Mentoring Department
Chemistry
Additional Mentoring Department
Biological Sciences
The Effects of 2-deoxy-D-glucose and Different E. coli Diets on C. elegans Mitochondrial Metabolism
SURC Ballroom C/D
We have investigated the effects of different bacterial diets on C. elegans as a model for metabolic studies in obesity and diabetes research. Our goal was to understand C. elegans’ base line metabolism by measuring ATP content under different dietary conditions. This study examined three strains of E. coli that can be used as food for C. elegans. The E. coli strains OP50 and HB101 are used as regular diets and HT115, which is a derivative of HB101. It has previously been shown that OP50 has higher fatty acid composition and HB101 has higher carbohydrate composition. The ATP content in OP50 fed worms was significantly lower than the HB101 and HT115 fed worms in both wild-type and nnt-1 strains of C. elegans. The nnt-1 strain is deficient in a mitochondrial protein, nicotinamide nucleotide transhydrogenase (NNT-1), associated with type-2 diabetes. In addition to these baseline studies, 2-deoxy-D-glucose was used as an inhibitor of glycolysis as a method for forcing fat metabolism in C. elegans. This dietary model could then be used to investigate the effects of increased fat metabolism on mitochondrial function in C. elegans. The baseline results were reversed in the 2-deoxy-D-glucose experiments suggesting that the worms were relying on fat metabolism. Future studies are planned to measure mitochondrial membrane potential, hydrogen peroxide production, and respiration to further understand the effects of increased fat metabolism on the mitochondria.
Faculty Mentor(s)
Carin Thomas, Lucinda Carnell