Title

Toxic Effects of common Pharmaceutical Contaminants on Daphnia pulex

Presenter Information

Al Okere

Document Type

Oral Presentation

Location

SURC Ballroom C/D

Start Date

16-5-2013

End Date

16-5-2013

Abstract

Human activity introduces a wide variety of chemical pollutants to our aquatic habitats, which makes it important to understand the effects of these pollutants on the organisms that live within them. The purpose of my study was to assess the effect of four of some of the most commonly present pharmaceutical compounds (Carbamazepine (CBZ), Diclofenac (DIC), 17α- ethinylestradiol (EE2), and Metoprolol (MET)) on Daphnia pulex, a keystone plankton species. I exposed individuals of a single clonal line of Daphnia to the four pharmaceuticals separately and together as a mixture in order to examine the resultant effects on mortality. CBZ, DIC and MET induced significant mortality at ecologically relevant doses, as did a mixture of all four pharmaceuticals. In a second, ongoing study, I am using a single cell gel electrophoresis (comet) assay to measure whether each compound induces DNA damage in Daphnia embryos. The toxic effects of these pharmaceuticals on organisms such as Daphnia have important implications because they may reflect effects on other eukaryotes, including humans.

Poster Number

30

Faculty Mentor(s)

Alison Scoville

Additional Mentoring Department

Biological Sciences

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May 16th, 8:20 AM May 16th, 10:50 AM

Toxic Effects of common Pharmaceutical Contaminants on Daphnia pulex

SURC Ballroom C/D

Human activity introduces a wide variety of chemical pollutants to our aquatic habitats, which makes it important to understand the effects of these pollutants on the organisms that live within them. The purpose of my study was to assess the effect of four of some of the most commonly present pharmaceutical compounds (Carbamazepine (CBZ), Diclofenac (DIC), 17α- ethinylestradiol (EE2), and Metoprolol (MET)) on Daphnia pulex, a keystone plankton species. I exposed individuals of a single clonal line of Daphnia to the four pharmaceuticals separately and together as a mixture in order to examine the resultant effects on mortality. CBZ, DIC and MET induced significant mortality at ecologically relevant doses, as did a mixture of all four pharmaceuticals. In a second, ongoing study, I am using a single cell gel electrophoresis (comet) assay to measure whether each compound induces DNA damage in Daphnia embryos. The toxic effects of these pharmaceuticals on organisms such as Daphnia have important implications because they may reflect effects on other eukaryotes, including humans.