Title

Effects of Cannabidiol on MCF-7 Human Breast Cancer Cell Proliferation, Metabolism, and Morphology

Document Type

Oral Presentation

Location

SURC Ballroom C/D

Start Date

16-5-2013

End Date

16-5-2013

Abstract

The use of cannabis for both recreational and medicinal purposes is not new or novel. Of the diverse class of compounds found in cannabis, cannabidiol (CBD) has been shown in previous studies to impede cancer cell growth. The ability of CBD to inhibit cancer cell proliferation has wide implications for both cannabis and cancer. The purpose of this study was to determine CBD’s ability to affect MCF-7 human breast cancer cell proliferation and morphology. Briefly, pure CBD was extracted using hexane in two simple chromatographic columns and used to treat subconfluent MCF-7 human breast cancer and C2C12 mouse myoblast cell cultures. MCF-7 and C2C12 cell lines were treated with standard solutions of 1, 2, 10, 50, 100, and 200 ug/mL CBD. MCF-7 and C2C12 metabolism and proliferation was quantitatively analyzed using MTT and hemacytometer counts, respectively and compared across all CBD concentrations. MCF-7 and C2C12 cell morphology was qualitatively evaluated using fluorescence and inverted light microscopy. Results showed that CBD-treated MCF-7 cancer cells experienced decreased proliferation, reduced size, and changed morphology, whereas C2C12 myoblast cells showed negligible decreases in proliferation and morphological changes. The implications of this study show a possible alternative use of CBD for treatment of cancer with reduced non-cancerous cell toxicity.

Poster Number

26

Faculty Mentor(s)

Ian Quitadamo

Additional Mentoring Department

Biological Sciences

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May 16th, 8:20 AM May 16th, 10:50 AM

Effects of Cannabidiol on MCF-7 Human Breast Cancer Cell Proliferation, Metabolism, and Morphology

SURC Ballroom C/D

The use of cannabis for both recreational and medicinal purposes is not new or novel. Of the diverse class of compounds found in cannabis, cannabidiol (CBD) has been shown in previous studies to impede cancer cell growth. The ability of CBD to inhibit cancer cell proliferation has wide implications for both cannabis and cancer. The purpose of this study was to determine CBD’s ability to affect MCF-7 human breast cancer cell proliferation and morphology. Briefly, pure CBD was extracted using hexane in two simple chromatographic columns and used to treat subconfluent MCF-7 human breast cancer and C2C12 mouse myoblast cell cultures. MCF-7 and C2C12 cell lines were treated with standard solutions of 1, 2, 10, 50, 100, and 200 ug/mL CBD. MCF-7 and C2C12 metabolism and proliferation was quantitatively analyzed using MTT and hemacytometer counts, respectively and compared across all CBD concentrations. MCF-7 and C2C12 cell morphology was qualitatively evaluated using fluorescence and inverted light microscopy. Results showed that CBD-treated MCF-7 cancer cells experienced decreased proliferation, reduced size, and changed morphology, whereas C2C12 myoblast cells showed negligible decreases in proliferation and morphological changes. The implications of this study show a possible alternative use of CBD for treatment of cancer with reduced non-cancerous cell toxicity.