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Campus where you would like to present
SURC Room 137B
Start Date
15-5-2014
End Date
15-5-2014
Keywords
Temperature-dependent sex determination, DMRT1, Gekko japonicus
Abstract
In this study, I observed how the DMRT1 gene affects Geckos with temperature-dependent sex determination during reptile development. To do this, I observed the rate of transcription of the DMRT1 gene in the Gecko species Gekko japonicus. Pregnant geckos were caught around Nanjing, China. After the females laid their eggs, the eggs were incubated at three different temperature regimes. For each temperature regime geckos were randomly placed in one of four different treatment groups. These groups included dissection at either oviposition, after 1/3 of development, or 2/3rds of development, and a hatching group, which were sexed at 120 days of age. Total RNA was extracted from the dissected embryos. Relative rates of transcription of the DMRT1 gene were assessed using quantitative PCR (qPCR).
Recommended Citation
Mullen, Lisa-Marie, "Temperature Sex Determination and the DMRT1 gene in Gekko japonicas" (2014). Symposium Of University Research and Creative Expression (SOURCE). 65.
https://digitalcommons.cwu.edu/source/2014/oralpresentations/65
Additional Mentoring Department
Biological Sciences
Temperature Sex Determination and the DMRT1 gene in Gekko japonicas
SURC Room 137B
In this study, I observed how the DMRT1 gene affects Geckos with temperature-dependent sex determination during reptile development. To do this, I observed the rate of transcription of the DMRT1 gene in the Gecko species Gekko japonicus. Pregnant geckos were caught around Nanjing, China. After the females laid their eggs, the eggs were incubated at three different temperature regimes. For each temperature regime geckos were randomly placed in one of four different treatment groups. These groups included dissection at either oviposition, after 1/3 of development, or 2/3rds of development, and a hatching group, which were sexed at 120 days of age. Total RNA was extracted from the dissected embryos. Relative rates of transcription of the DMRT1 gene were assessed using quantitative PCR (qPCR).
Faculty Mentor(s)
Sun, Lixing