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Presenter Information

Kaitlin Koppinger

Location

SURC Room 137B

Start Date

15-5-2014

End Date

15-5-2014

Keywords

Hookworm, natural plant extract, anthelmintic

Abstract

Approximately one billion people world-wide are infected with hookworms, intestinal parasites that cause a multitude of harmful symptoms including anemia. Studies have shown that hookworms are becoming resistant to current drugs. The goal of this project was to assess the anthelmintic effects of natural plant extracts and their chromatographic enriched fractions against the hookworm Ancylostoma ceylanicum. Syrian hamsters were used as the model host to complete the hookworm life cycle. Testing of these extracts and fractions on the adult hookworm was done using an ex vivo assay. Data showed that whole extracts and fractions of the plant Dalea ornata, actively decreased motility or induced mortality of adult hookworm ex vivo. Fractions were tested at three concentrations of 100, 50, and 10ug/ml. These three doses of the fraction showed anthelminthic activity with the lowest concentration, 10ug/ml, recording 0 percent worm survival by Day 5 of exposure.

Faculty Mentor(s)

Dondji, Blaise

Additional Mentoring Department

Biological Sciences

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May 15th, 1:30 PM May 15th, 1:50 PM

Northwestern United States plant extracts show anthelminthic activity against the hookworm, Ancylostoma ceylanicum

SURC Room 137B

Approximately one billion people world-wide are infected with hookworms, intestinal parasites that cause a multitude of harmful symptoms including anemia. Studies have shown that hookworms are becoming resistant to current drugs. The goal of this project was to assess the anthelmintic effects of natural plant extracts and their chromatographic enriched fractions against the hookworm Ancylostoma ceylanicum. Syrian hamsters were used as the model host to complete the hookworm life cycle. Testing of these extracts and fractions on the adult hookworm was done using an ex vivo assay. Data showed that whole extracts and fractions of the plant Dalea ornata, actively decreased motility or induced mortality of adult hookworm ex vivo. Fractions were tested at three concentrations of 100, 50, and 10ug/ml. These three doses of the fraction showed anthelminthic activity with the lowest concentration, 10ug/ml, recording 0 percent worm survival by Day 5 of exposure.