Title

Assessment of Anthelminthic Activity of Plant Extracts on Ancylostoma Ceylanicum and the Development of a Toxicity Bioassay

Presenter Information

Jocelyn McCornack

Document Type

Oral Presentation

Location

SURC 140

Start Date

21-5-2015

End Date

21-5-2015

Keywords

Hookworm, Natural Products, Toxicity Testing

Abstract

Soil-transmitted helminthes are intestinal parasites that contribute to major disease burden in people within tropical and impoverished areas. It has been estimated this blood sucking parasite has been responsible for the loss of over one million liters of blood per day. Recently, resistance to current control programs has rendered it necessary to develop new treatments. Earlier projects within the lab have assessed the anthelmintic effects of plant extracts and their enriched fractions on Ancylostoma ceylanicum ex vivo. The plants Dalea ornata and Oemlaria cerasiformis have shown efficacy with adult worms showing either significant decreases in motility or mortality. The additional fractions tested, however, did not display anthelminthic activity. Another important step was to develop an assay testing the toxicity of the extracts to mammalian cells. Three were attempted: a Trypan Blue assay, a Flow Cytometer assay, and a Cell Counting Kit assay. All of them used hamster splenocytes with the extracts at various concentrations. Death and mitosis were monitored over a three to five day period and compared with the control.

Faculty Mentor(s)

Blaise Dondji

Department/Program

Biological Sciences

Additional Mentoring Department

Biological Sciences

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May 21st, 2:40 PM May 21st, 3:00 PM

Assessment of Anthelminthic Activity of Plant Extracts on Ancylostoma Ceylanicum and the Development of a Toxicity Bioassay

SURC 140

Soil-transmitted helminthes are intestinal parasites that contribute to major disease burden in people within tropical and impoverished areas. It has been estimated this blood sucking parasite has been responsible for the loss of over one million liters of blood per day. Recently, resistance to current control programs has rendered it necessary to develop new treatments. Earlier projects within the lab have assessed the anthelmintic effects of plant extracts and their enriched fractions on Ancylostoma ceylanicum ex vivo. The plants Dalea ornata and Oemlaria cerasiformis have shown efficacy with adult worms showing either significant decreases in motility or mortality. The additional fractions tested, however, did not display anthelminthic activity. Another important step was to develop an assay testing the toxicity of the extracts to mammalian cells. Three were attempted: a Trypan Blue assay, a Flow Cytometer assay, and a Cell Counting Kit assay. All of them used hamster splenocytes with the extracts at various concentrations. Death and mitosis were monitored over a three to five day period and compared with the control.