Title

Observing the Effects of Novel Flavonoid Malheuran-2 on MCF-7 Cells Using Flow Cytometric Analysis

Presenter Information

Dean Hoffer

Document Type

Oral Presentation

Location

SURC Ballroom B/C/D

Start Date

21-5-2015

End Date

21-5-2015

Keywords

Flow Cytometry, Cancer, Cell Biology

Abstract

Apoptosis is the mechanism used to regulate the balance between cell proliferation and cell death. Reduced levels of apoptosis or increased levels of proliferation can lead to regions of uncontrollable growth, or cancer. Flavonoids are being studied because of their anti-tumor qualities and low toxicity on surrounding healthy tissue. Of six flavonoids extracted from the Dalea searlsiae plant, flavonoids 1 to 4 showed inhibitory effects on the proliferation of MCF-7 cells. After further studying the effects of flavonoid 2, apoptosis was confirmed using fluorescent microscopy and staining with Annexin V Alexa fluor 488 and propidium iodide. Analysis using flow cytometry was impossible due to false binding of Annexin which was attributed to harsh cell harvesting techniques compromising the cell membrane and surface proteins.

Poster Number

45

Faculty Mentor(s)

Eric Foss

Department/Program

Biological Sciences

Additional Mentoring Department

Biological Sciences

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May 21st, 8:30 AM May 21st, 11:00 AM

Observing the Effects of Novel Flavonoid Malheuran-2 on MCF-7 Cells Using Flow Cytometric Analysis

SURC Ballroom B/C/D

Apoptosis is the mechanism used to regulate the balance between cell proliferation and cell death. Reduced levels of apoptosis or increased levels of proliferation can lead to regions of uncontrollable growth, or cancer. Flavonoids are being studied because of their anti-tumor qualities and low toxicity on surrounding healthy tissue. Of six flavonoids extracted from the Dalea searlsiae plant, flavonoids 1 to 4 showed inhibitory effects on the proliferation of MCF-7 cells. After further studying the effects of flavonoid 2, apoptosis was confirmed using fluorescent microscopy and staining with Annexin V Alexa fluor 488 and propidium iodide. Analysis using flow cytometry was impossible due to false binding of Annexin which was attributed to harsh cell harvesting techniques compromising the cell membrane and surface proteins.