Document Type
Thesis
Date of Degree Completion
Spring 2024
Degree Name
Master of Science (MS)
Department
Biology
Committee Chair
Linda Raubeson
Second Committee Member
Dan Beck
Third Committee Member
Alison Scoville
Fourth Committee Member
N/A
Abstract
Ranunculus triternatus, a rare endemic species of the Columbia River Gorge, faces conservation challenges due to its limited range and fragmented populations. This study assesses the genetic diversity of R. triternatus and compares it with its widespread congener, Ranunculus glaberrimus, using inter-primer binding site (iPBS) markers. Genetic data from 80 individuals across eight populations were analyzed using Nei’s genetic diversity, Shannon’s diversity index, and AMOVA. Results showed R. triternatus had higher genetic diversity than western populations of R. glaberrimus, with eastern populations of R. glaberrimus displaying greater diversity than western ones. PCA, UPGMA, and STRUCTURE analysis revealed genetic relationships and potential admixture between populations. Despite its restricted range, R. triternatus exhibited significant genetic resilience, suggesting genetic health is not the primary cause of its decline. Conservation efforts should focus on habitat protection and expansion. This study underscores the importance of genetic diversity in conservation planning and provides a foundation for future research on the genetic diversity and ecology of both Ranunculus species.
Recommended Citation
Garcia, Ryan, "The Genetic Diversity of an Endangered Buttercup, Ranunculus triternatus" (2024). All Master's Theses. 1946.
https://digitalcommons.cwu.edu/etd/1946
Included in
Genetics Commons, Other Ecology and Evolutionary Biology Commons, Plant Breeding and Genetics Commons, Population Biology Commons