Title

Identification of Proteins that interact with QuakingI-6

Document Type

Oral Presentation

Campus where you would like to present

Ellensburg

Event Website

https://digitalcommons.cwu.edu/source

Start Date

15-5-2019

End Date

15-5-2019

Abstract

The mammalian neocortex is the brain structure that mediates conscious thoughts and actions and is the source of human intelligence. This structure is divided into discrete areas that carry out different computational tasks. Neocortical arealization is the process through which the specific areas of the neocortex are formed, and occurs during embryonic development. In order to better understand this process, it is helpful to understand how the proteins that regulate neuronal development interact with each other. Emx2 plays an important role in controlling the size of the functional areas within the developing neocortex, the brain structure responsible for conscious thought, and interacts with each of the isoforms of a protein called QuakingI. The goal of this project is to identify proteins that interact with QuakingI-6 so to determine how it mechanistically influences neocortical arealization. To identify proteins to which QuakingI-6 interacts, a yeast two-hybrid screen was performed using QuakingI-6 as bait with a protein library. The plasmids encoding the prey proteins were rescued from the yeast cells and sequenced to identify the proteins interacting with QuakingI-6. The main proteins identified from this screen so far are β-actin, Receptor for activated kinase 1 (Rack1), and Transducin-like enhancer of split (Tle2). β-actin is a cytoskeletal protein that has many roles in the body including the developing neocortex, Rack1 is a scaffold protein that interacts with multiple cell signaling pathway, and Tle2 is a transcriptional repressor. At this point there are more assays that need to be performed to confirm these interactions.

Faculty Mentor(s)

Todd Kroll

Department/Program

Chemistry

SOURCE_Moreno.pptx (7813 kB)
Slides for SOURCE 2019 presentation Leon

Additional Files

SOURCE_Moreno.pptx (7813 kB)
Slides for SOURCE 2019 presentation Leon

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May 15th, 12:00 AM May 15th, 12:00 AM

Identification of Proteins that interact with QuakingI-6

Ellensburg

The mammalian neocortex is the brain structure that mediates conscious thoughts and actions and is the source of human intelligence. This structure is divided into discrete areas that carry out different computational tasks. Neocortical arealization is the process through which the specific areas of the neocortex are formed, and occurs during embryonic development. In order to better understand this process, it is helpful to understand how the proteins that regulate neuronal development interact with each other. Emx2 plays an important role in controlling the size of the functional areas within the developing neocortex, the brain structure responsible for conscious thought, and interacts with each of the isoforms of a protein called QuakingI. The goal of this project is to identify proteins that interact with QuakingI-6 so to determine how it mechanistically influences neocortical arealization. To identify proteins to which QuakingI-6 interacts, a yeast two-hybrid screen was performed using QuakingI-6 as bait with a protein library. The plasmids encoding the prey proteins were rescued from the yeast cells and sequenced to identify the proteins interacting with QuakingI-6. The main proteins identified from this screen so far are β-actin, Receptor for activated kinase 1 (Rack1), and Transducin-like enhancer of split (Tle2). β-actin is a cytoskeletal protein that has many roles in the body including the developing neocortex, Rack1 is a scaffold protein that interacts with multiple cell signaling pathway, and Tle2 is a transcriptional repressor. At this point there are more assays that need to be performed to confirm these interactions.

https://digitalcommons.cwu.edu/source/2019/Oralpres/82