Quantitative Application for SDS–PAGE in a Biochemistry Lab
Department or Administrative Unit
A variety of laboratory experiments have been published in this Journal describing the use of polyacrylamide gel electrophoresis to investigate different properties of proteins, including determination of the subunit composition of individual proteins and molar mass. The experiment described extends the capabilities of this experimental technique and provides a mechanism to determine the concentration of a single protein from a heterogeneous mixture.
Carter, J., Petersen, B. P., Printz, S. A., Sorey, T. L., & Kroll, T. T. (2013). Quantitative Application for SDS–PAGE in a Biochemistry Lab. Journal of Chemical Education, 90(9), 1255–1256. https://doi.org/10.1021/ed300390j
Journal of Chemical Education
Copyright © 2013 The American Chemical Society and Division of Chemical Education, Inc.