Inhibition by orthophosphate esters of glucose-6-phosphatase
Department or Administrative Unit
Inhibition of rat liver microsomal glucose-6-phosphatase (D-glucose-6-phosphate phosphohydrolase, EC 126.96.36.199) by orthophosphate and organophosphate esters was examined at pH 6.0 and 7.5 with and without enzyme pretreatment with 0.2% (w/v) deoxycholate. Inhibition by orthophosphate and monoethyl phosphate was competitive with respect to glucose-6-P while inhibition by mono- and di-phenyl phosphate was of the mixed type. Monoalkyl phosphates were more effective inhibitors than the analogous di- and tri-alkyl phosphates and deoxycholate potentiated the inhibitory effects. Mono- and di-phenyl phosphates were more effective inhibitors than triphenyl phosphate, and deoxycholate decreased these inhibitory effects. The results are interpreted in terms of inhibitor and deoxycholate interactions with the enzyme.
Walls, M. A., & Lygre, D. G. (1980). Inhibition by orthophosphate esters of glucose-6-phosphatase. Canadian Journal of Biochemistry, 58(9), 673–676. https://doi.org/10.1139/o80-094
Canadian Journal of Biochemistry