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Presenter Information

Lisa-Marie Mullen

Location

SURC Room 137B

Start Date

15-5-2014

End Date

15-5-2014

Keywords

Temperature-dependent sex determination, DMRT1, Gekko japonicus

Abstract

In this study, I observed how the DMRT1 gene affects Geckos with temperature-dependent sex determination during reptile development. To do this, I observed the rate of transcription of the DMRT1 gene in the Gecko species Gekko japonicus. Pregnant geckos were caught around Nanjing, China. After the females laid their eggs, the eggs were incubated at three different temperature regimes. For each temperature regime geckos were randomly placed in one of four different treatment groups. These groups included dissection at either oviposition, after 1/3 of development, or 2/3rds of development, and a hatching group, which were sexed at 120 days of age. Total RNA was extracted from the dissected embryos. Relative rates of transcription of the DMRT1 gene were assessed using quantitative PCR (qPCR).

Faculty Mentor(s)

Sun, Lixing

Additional Mentoring Department

Biological Sciences

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May 15th, 12:20 PM May 15th, 12:40 PM

Temperature Sex Determination and the DMRT1 gene in Gekko japonicas

SURC Room 137B

In this study, I observed how the DMRT1 gene affects Geckos with temperature-dependent sex determination during reptile development. To do this, I observed the rate of transcription of the DMRT1 gene in the Gecko species Gekko japonicus. Pregnant geckos were caught around Nanjing, China. After the females laid their eggs, the eggs were incubated at three different temperature regimes. For each temperature regime geckos were randomly placed in one of four different treatment groups. These groups included dissection at either oviposition, after 1/3 of development, or 2/3rds of development, and a hatching group, which were sexed at 120 days of age. Total RNA was extracted from the dissected embryos. Relative rates of transcription of the DMRT1 gene were assessed using quantitative PCR (qPCR).