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Location

SURC Room 137B

Start Date

15-5-2014

End Date

15-5-2014

Keywords

Chromatography, Spectroscopy, Chemistry

Abstract

Under the principal investigator Dr. Gil Belofsky of the Department of Chemistry, this research project is focused on the discovery and application of novel compounds from the plant Dalea ornata, commonly known as the Blue Mountain prairie clover. The process of isolating each compound was accomplished through techniques such as Sephadex LH-20 size exclusion chromatography and linear continuous gradient chromatography over silica gel. Eight different phenolic compounds have been isolated, with several more nearly pure. Advanced nuclear magnetic resonance spectroscopy techniques were utilized to elucidate the structure of each isolated compound. In the event that additional amounts are needed, re-isolation of compounds will be performed for expanded biological testing. Initial ex vivo biological testing of D. ornata extracts have demonstrated anthelmintic activity towards the hookworm Ancylostoma ceylanicum. The World Health Organization projects that one billion individuals are currently infected with hookworms or related soil-transmitted, parasitic nematodes. While medications are available to treat acute cases, there is no current treatment to prevent hookworm infection. Determination of the D. ornata compound(s) associated with the observed anthelmintic activity will likely provide further insight into mechanisms of inhibiting and preventing hookworm infections.

Faculty Mentor(s)

Belofsky, Gil

Additional Mentoring Department

Chemistry

Additional Mentoring Department

Biology

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May 15th, 1:10 PM May 15th, 1:30 PM

Application of Dalea ornata (Fabaceae) extractives toward inhibition of the hookworm Ancylostoma ceylanicum

SURC Room 137B

Under the principal investigator Dr. Gil Belofsky of the Department of Chemistry, this research project is focused on the discovery and application of novel compounds from the plant Dalea ornata, commonly known as the Blue Mountain prairie clover. The process of isolating each compound was accomplished through techniques such as Sephadex LH-20 size exclusion chromatography and linear continuous gradient chromatography over silica gel. Eight different phenolic compounds have been isolated, with several more nearly pure. Advanced nuclear magnetic resonance spectroscopy techniques were utilized to elucidate the structure of each isolated compound. In the event that additional amounts are needed, re-isolation of compounds will be performed for expanded biological testing. Initial ex vivo biological testing of D. ornata extracts have demonstrated anthelmintic activity towards the hookworm Ancylostoma ceylanicum. The World Health Organization projects that one billion individuals are currently infected with hookworms or related soil-transmitted, parasitic nematodes. While medications are available to treat acute cases, there is no current treatment to prevent hookworm infection. Determination of the D. ornata compound(s) associated with the observed anthelmintic activity will likely provide further insight into mechanisms of inhibiting and preventing hookworm infections.