Ethanol as a solvent in pharmaceutical toxicity testing should be used with caution
Document Type
Poster
Campus where you would like to present
Ellensburg
Event Website
https://digitalcommons.cwu.edu/source
Start Date
18-5-2020
Abstract
Mouse liver cells have a vital role in many scientific fields as they are used to test for toxicity of pharmaceutical compounds. Additionally, ethanol is usually used as the vehicle control to dissolve those compounds. Different concentrations of ethanol were investigated to find the optimal amount to be used for cells grown in vitro. Vector transfected Hepa-V mouse liver cells were grown in 25 cm2 flasks using a complete media solution containing DMEM/F12, Nu-Serum and 1% Penicillin-Streptomycin. After reaching 80% confluency, the cells were exposed to either the vehicle control (0.83 to 3.34% of ethanol) or the xenobiotics (5 to 20 μM of Antimycin A) for 24 hours. WST-8 was added to the cells and absorbance of the solution was measured at 450 nm using Synergy 2 plate reader. The readouts showed a decreasing trend in the amount of living cells in both final solutions of vehicle control treatment and xenobiotics treatment. However, those treated with just ethanol observed a more linear relationship (R2 = 0.96) compared to those exposed to Antimycin A alone (R2 = 0.68). Cell viability of cells treated with Antimycin A reached a threshold after 10 μM while those introduced to ethanol experienced more cell death as percent of ethanol in the vehicle control increased. This results confirm that ethanol has a noticeable effect on cell viability and it should be used no higher than 2.5% in cell culture.
Recommended Citation
Luong, Phuong, "Ethanol as a solvent in pharmaceutical toxicity testing should be used with caution" (2020). Symposium Of University Research and Creative Expression (SOURCE). 36.
https://digitalcommons.cwu.edu/source/2020/COTS/36
Department/Program
Chemistry
Additional Mentoring Department
https://cwu.studentopportunitycenter.com/2020/04/ethanol-as-a-solvent-in-pharmaceutical-toxicity-testing-should-be-used-with-caution/
Ethanol as a solvent in pharmaceutical toxicity testing should be used with caution
Ellensburg
Mouse liver cells have a vital role in many scientific fields as they are used to test for toxicity of pharmaceutical compounds. Additionally, ethanol is usually used as the vehicle control to dissolve those compounds. Different concentrations of ethanol were investigated to find the optimal amount to be used for cells grown in vitro. Vector transfected Hepa-V mouse liver cells were grown in 25 cm2 flasks using a complete media solution containing DMEM/F12, Nu-Serum and 1% Penicillin-Streptomycin. After reaching 80% confluency, the cells were exposed to either the vehicle control (0.83 to 3.34% of ethanol) or the xenobiotics (5 to 20 μM of Antimycin A) for 24 hours. WST-8 was added to the cells and absorbance of the solution was measured at 450 nm using Synergy 2 plate reader. The readouts showed a decreasing trend in the amount of living cells in both final solutions of vehicle control treatment and xenobiotics treatment. However, those treated with just ethanol observed a more linear relationship (R2 = 0.96) compared to those exposed to Antimycin A alone (R2 = 0.68). Cell viability of cells treated with Antimycin A reached a threshold after 10 μM while those introduced to ethanol experienced more cell death as percent of ethanol in the vehicle control increased. This results confirm that ethanol has a noticeable effect on cell viability and it should be used no higher than 2.5% in cell culture.
https://digitalcommons.cwu.edu/source/2020/COTS/36
Faculty Mentor(s)
Carin Thomas