Aging is Associated with a Downregulation of Genes Linked to Protein Ubiquitination and Glucose Metabolism in Human Skeletal Muscle
Document Type
Poster
Event Website
https://source2022.sched.com/
Start Date
16-5-2022
End Date
16-5-2022
Keywords
Muscle, Aging, Downregulation
Abstract
Skeletal muscle is known to change through the natural course of aging. However, our understanding of which specific biological processes may contribute to these changes are not fully understood. PURPOSE: To identify biological processes in aging skeletal muscle that are associated with genes downregulated in the skeletal muscle of older adults. METHODS: Skeletal muscle biopsy samples (vastus lateralis) were analyzed from healthy younger (27±3yr; 8M, 1F; BMI: 24.5±2.0) and older adults (68±5yr; 6M, 3F; BMI: 25.9±4.7 kg•m^(-2)). Muscle biopsies were obtained after an overnight fast under resting conditions and controlling for physical activity. Whole transcriptome next-generation sequencing (HISeq2500, Illumina) was performed on cDNA produced from isolated RNA. Differential gene expression between young and old was identified (with young as reference) using an adjusted p-value of
Recommended Citation
Hart, Colton B., "Aging is Associated with a Downregulation of Genes Linked to Protein Ubiquitination and Glucose Metabolism in Human Skeletal Muscle" (2022). Symposium Of University Research and Creative Expression (SOURCE). 27.
https://digitalcommons.cwu.edu/source/2022/CEPS/27
Department/Program
Exercise Science
Additional Mentoring Department
Health Sciences
Aging is Associated with a Downregulation of Genes Linked to Protein Ubiquitination and Glucose Metabolism in Human Skeletal Muscle
Skeletal muscle is known to change through the natural course of aging. However, our understanding of which specific biological processes may contribute to these changes are not fully understood. PURPOSE: To identify biological processes in aging skeletal muscle that are associated with genes downregulated in the skeletal muscle of older adults. METHODS: Skeletal muscle biopsy samples (vastus lateralis) were analyzed from healthy younger (27±3yr; 8M, 1F; BMI: 24.5±2.0) and older adults (68±5yr; 6M, 3F; BMI: 25.9±4.7 kg•m^(-2)). Muscle biopsies were obtained after an overnight fast under resting conditions and controlling for physical activity. Whole transcriptome next-generation sequencing (HISeq2500, Illumina) was performed on cDNA produced from isolated RNA. Differential gene expression between young and old was identified (with young as reference) using an adjusted p-value of
https://digitalcommons.cwu.edu/source/2022/CEPS/27
Faculty Mentor(s)
Jared Dickinson, Jon Dickinson