Title

Molecular Motor Movement in a 3D Cytoskeleton Network

Document Type

Poster

Event Website

https://source2022.sched.com/

Start Date

16-5-2022

End Date

16-5-2022

Keywords

Biophysics, Computational, Physics

Abstract

The cytoskeleton of a cell is composed of microtubules, actin filaments, and intermediate filaments. Cargo, such as organelles and protein complexes, is transported around the cytoskeleton by molecular motors such as kinesin and dynein. The filament network can vary wildly from cell to cell, so modeling molecular motor movement can be challenging. The goal of this project is to use computational simulations to model the movement of multiple molecular motors within a cytoskeletal network. In order to simulate the movement of molecular motors, a model for the internal structure of the cell was first needed. To account for the variations between cells, a cubic array is established with multiple actin and microtubule filaments randomly placed within each layer of the array. A modified 3-dimensional random walk program is used to simulate the biased Brownian movement of molecular motor complexes through a cell. The bias of the Brownian motion is applied based on the molecular motor’s current position within the cytoskeletal network. Initial simulations show that molecular motor complexes will move along the filaments for the most part, but will eventually detach and undergo pure Brownian motion until they latch onto another filament.

Faculty Mentor(s)

Erin Craig

Department/Program

Physics

Additional Mentoring Department

Physics

Streaming Media

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May 16th, 12:00 AM May 16th, 12:00 AM

Molecular Motor Movement in a 3D Cytoskeleton Network

The cytoskeleton of a cell is composed of microtubules, actin filaments, and intermediate filaments. Cargo, such as organelles and protein complexes, is transported around the cytoskeleton by molecular motors such as kinesin and dynein. The filament network can vary wildly from cell to cell, so modeling molecular motor movement can be challenging. The goal of this project is to use computational simulations to model the movement of multiple molecular motors within a cytoskeletal network. In order to simulate the movement of molecular motors, a model for the internal structure of the cell was first needed. To account for the variations between cells, a cubic array is established with multiple actin and microtubule filaments randomly placed within each layer of the array. A modified 3-dimensional random walk program is used to simulate the biased Brownian movement of molecular motor complexes through a cell. The bias of the Brownian motion is applied based on the molecular motor’s current position within the cytoskeletal network. Initial simulations show that molecular motor complexes will move along the filaments for the most part, but will eventually detach and undergo pure Brownian motion until they latch onto another filament.

https://digitalcommons.cwu.edu/source/2022/COTS/53