Document Type


Date of Degree Completion

Fall 2020

Degree Name

Bachelor of Science



Committee Chair

Gil Belofsky

Second Committee Member

JoAnn Peters

Third Committee Member

Allyson Rogan-Klyve


The plant genus Dalea (Fabaceae) has been found to be prolific in its production of phenolic secondary metabolites including flavonoids, isoflavonoids, chalcones, pterocarpans, stilbenes, and other biosynthetically related compounds. Biological testing has revealed wide-ranging activities for these metabolites in many prior reports. Dalea jamesii has not yet been investigated for its chemical content. This study will be conducted with the hypothesis that unique phenolic secondary metabolites will be produced in the roots of D. jamesii. It will be of interest to determine if the isolated metabolites exhibit biomedically related activities. It will also be of interest to investigate whether the phenolic secondary metabolites show any bioactivities.

Using various techniques, including vacuum liquid chromatography, Sephadex LH-20 chromatography, and linear gradient or step gradient chromatography, the secondary metabolites from the root portion of D. jamesii will be separated. Thin-layer chromatography (TLC) and 1H NMR spectroscopy will be used to combine similar materials for further purification. Structure determination of the pure compounds will be accomplished primarily by extensive 1D and 2D NMR spectroscopy.