Title

Dalea searlsiae

Presenter Information

Mario Aronica

Document Type

Oral Presentation

Location

SURC Ballroom A

Start Date

17-5-2012

End Date

17-5-2012

Abstract

This research involves the study of natural products chemistry. The goal is to isolate and characterize new compounds from the plant Dalea searlsiae to test for antimicrobial activity, specifically again Staphylococcus aureus. S. aureus is a bacterium that can develop multidrug resistance and is a developing problem, with limited treatment options. In order to overcome multidrug resistance in the cells, natural products can be mixed with a known antibiotic and both are added to the cells. The cells have transporters within their walls that pump compounds in and out of the cell. The drug penetrates the cell and, in this scenario, the pump inhibitor can stop the cell from pumping out the drug, which then can more effectively kill the cell. The components of the aerial parts of the plant were extracted then characterized and analyzed. Chromatography is a refinement process consisting of successive stages, taking crude mixtures to pure compounds. Some of the general experimental procedures include thin layer chromatography (TLC), size exclusion chromatography, and linear solvent gradient chromatography. The structure elucidation of pure compounds is accomplished by nuclear magnetic resonance spectroscopy (NMR) and mass spectrometry. To date, six pure compounds have been isolated from D. searlsiae. NMR spectra were obtained for the compounds and biological testing is currently in progress.

Poster Number

22

Faculty Mentor(s)

Gil Belofsky

Additional Mentoring Department

Chemistry

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May 17th, 8:30 AM May 17th, 11:00 AM

Dalea searlsiae

SURC Ballroom A

This research involves the study of natural products chemistry. The goal is to isolate and characterize new compounds from the plant Dalea searlsiae to test for antimicrobial activity, specifically again Staphylococcus aureus. S. aureus is a bacterium that can develop multidrug resistance and is a developing problem, with limited treatment options. In order to overcome multidrug resistance in the cells, natural products can be mixed with a known antibiotic and both are added to the cells. The cells have transporters within their walls that pump compounds in and out of the cell. The drug penetrates the cell and, in this scenario, the pump inhibitor can stop the cell from pumping out the drug, which then can more effectively kill the cell. The components of the aerial parts of the plant were extracted then characterized and analyzed. Chromatography is a refinement process consisting of successive stages, taking crude mixtures to pure compounds. Some of the general experimental procedures include thin layer chromatography (TLC), size exclusion chromatography, and linear solvent gradient chromatography. The structure elucidation of pure compounds is accomplished by nuclear magnetic resonance spectroscopy (NMR) and mass spectrometry. To date, six pure compounds have been isolated from D. searlsiae. NMR spectra were obtained for the compounds and biological testing is currently in progress.