Dalea searlsiae
Document Type
Oral Presentation
Campus where you would like to present
SURC Ballroom A
Start Date
17-5-2012
End Date
17-5-2012
Abstract
This research involves the study of natural products chemistry. The goal is to isolate and characterize new compounds from the plant Dalea searlsiae to test for antimicrobial activity, specifically again Staphylococcus aureus. S. aureus is a bacterium that can develop multidrug resistance and is a developing problem, with limited treatment options. In order to overcome multidrug resistance in the cells, natural products can be mixed with a known antibiotic and both are added to the cells. The cells have transporters within their walls that pump compounds in and out of the cell. The drug penetrates the cell and, in this scenario, the pump inhibitor can stop the cell from pumping out the drug, which then can more effectively kill the cell. The components of the aerial parts of the plant were extracted then characterized and analyzed. Chromatography is a refinement process consisting of successive stages, taking crude mixtures to pure compounds. Some of the general experimental procedures include thin layer chromatography (TLC), size exclusion chromatography, and linear solvent gradient chromatography. The structure elucidation of pure compounds is accomplished by nuclear magnetic resonance spectroscopy (NMR) and mass spectrometry. To date, six pure compounds have been isolated from D. searlsiae. NMR spectra were obtained for the compounds and biological testing is currently in progress.
Recommended Citation
Aronica, Mario, "Dalea searlsiae" (2012). Symposium Of University Research and Creative Expression (SOURCE). 8.
https://digitalcommons.cwu.edu/source/2012/posters/8
Poster Number
22
Additional Mentoring Department
Chemistry
Dalea searlsiae
SURC Ballroom A
This research involves the study of natural products chemistry. The goal is to isolate and characterize new compounds from the plant Dalea searlsiae to test for antimicrobial activity, specifically again Staphylococcus aureus. S. aureus is a bacterium that can develop multidrug resistance and is a developing problem, with limited treatment options. In order to overcome multidrug resistance in the cells, natural products can be mixed with a known antibiotic and both are added to the cells. The cells have transporters within their walls that pump compounds in and out of the cell. The drug penetrates the cell and, in this scenario, the pump inhibitor can stop the cell from pumping out the drug, which then can more effectively kill the cell. The components of the aerial parts of the plant were extracted then characterized and analyzed. Chromatography is a refinement process consisting of successive stages, taking crude mixtures to pure compounds. Some of the general experimental procedures include thin layer chromatography (TLC), size exclusion chromatography, and linear solvent gradient chromatography. The structure elucidation of pure compounds is accomplished by nuclear magnetic resonance spectroscopy (NMR) and mass spectrometry. To date, six pure compounds have been isolated from D. searlsiae. NMR spectra were obtained for the compounds and biological testing is currently in progress.
Faculty Mentor(s)
Gil Belofsky