Probing bacterial cell biology using image cytometry
Document Type
Article
Department or Administrative Unit
Physics
Publication Date
12-9-2016
Abstract
Advances in automated fluorescence microscopy have made snapshot and time‐lapse imaging of bacterial cells commonplace, yet fundamental challenges remain in analysis. The vast quantity of data collected in high‐throughput experiments requires a fast and reliable automated method to analyze fluorescence intensity and localization, cell morphology and proliferation as well as other descriptors. Inspired by effective yet tractable methods of population‐level analysis using flow cytometry, we have developed a framework and tools for facilitating analogous analyses in image cytometry. These tools can both visualize and gate (generate subpopulations) more than 70 cell descriptors, including cell size, age and fluorescence. The method is well suited to multi‐well imaging, analysis of bacterial cultures with high cell density (thousands of cells per frame) and complete cell cycle imaging. We give a brief description of the analysis of four distinct applications to emphasize the broad applicability of the tool.
Recommended Citation
Cass, J.A., Stylianidou, S., Kuwada, N.J., Traxler, B. and Wiggins, P.A. (2017), Probing bacterial cell biology using image cytometry. Molecular Microbiology, 103: 818-828. doi:10.1111/mmi.13591
Journal
Molecular Microbiology
Rights
© 2016 John Wiley & Sons Ltd
Comments
This article was originally published in Molecular Microbiology. The full-text article from the publisher can be found here.
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