Document Type
Thesis
Date of Degree Completion
Spring 1973
Degree Name
Master of Science (MS)
Department
Biology
Committee Chair
Robert Pacha
Second Committee Member
G. Clark
Third Committee Member
Thomas H. Thelen
Abstract
Two bacteriophages which infect the myxobacterium Cytophaga succinicans, strains 14 and 16, were isolated from fish hatchery effluent. Each was submitted to a detailed study. These phages have a limited host range, even within the genus Cytophaga. The turbid plaques formed by these phages are similar in appearance to those produced by coliphage Lambda, and average 0.85 mm in diameter. Both phages have a latent period of 195 minutes and a rise period of an additional 165 minutes. Phage 0CS-16 has a burst size of 137 and phage 0CS-14 has a burst of 45. Sodium ion at a concentration of 0.01 M acts as the optimum adsorption cofactor for 0CS-16, whereas Ammonium ion at 0.0001 M is optimum for 0CS-14. Both phages are viable at temperatures between 5 degrees Celsius and 25 degrees Celsius, but are inactivated when frozen. Both can withstand pH's between 6 and 9. Phage 0CS-14 is resistant to chloroform treatment for eight hours, whereas the titer of phage 0CS-16 decreases by nearly two log units within this time.
Recommended Citation
Lee, Kenneth Wayne, "Isolation and Characterization of Two Bacteriophages Infecting the Myxobacterium Cytophaga Succinicans" (1973). All Master's Theses. 2132.
https://digitalcommons.cwu.edu/etd/2132