The effect of the fullerene nanoparticle on mitochondrial respiration
Document Type
Oral Presentation
Campus where you would like to present
SURC Ballroom C/D
Start Date
16-5-2013
End Date
16-5-2013
Abstract
C60, or fullerenes, are a controversial nanoparticle because of their unknown effects to biological systems. With increased research into many different industrial and medical applications for C60, such as commercial lubricants and medical delivery devices, scientists are concerned of the potential adverse effects these nanoparticles could have on human health. We investigated the potential adverse effects of C60 on respiration of bovine heart mitochondria by monitoring oxygen consumption in the presence of NADH. C60 was solubilized in 7.5 percent Bovine Serum Albumin to keep the nanoparticles from clumping. C60 was used in concentrations ranging from 2 to 35 parts per million and incubated with bovine heart mitochondria for up to 10 minutes before measuring oxygen consumption. The rate of oxygen consumption was collected using a Clark-electrode and the data were analyzed using MS Excel. Preliminary results show that oxygen consumption from bovine heart mitochondria in the presence of NADH (Complexes I, III and IV of the electron transport chain) was affected by different concentrations of C60 or time of incubation with C60.
Recommended Citation
Miller, Zachary, "The effect of the fullerene nanoparticle on mitochondrial respiration" (2013). Symposium Of University Research and Creative Expression (SOURCE). 125.
https://digitalcommons.cwu.edu/source/2013/posters/125
Poster Number
40
Additional Mentoring Department
Chemistry
The effect of the fullerene nanoparticle on mitochondrial respiration
SURC Ballroom C/D
C60, or fullerenes, are a controversial nanoparticle because of their unknown effects to biological systems. With increased research into many different industrial and medical applications for C60, such as commercial lubricants and medical delivery devices, scientists are concerned of the potential adverse effects these nanoparticles could have on human health. We investigated the potential adverse effects of C60 on respiration of bovine heart mitochondria by monitoring oxygen consumption in the presence of NADH. C60 was solubilized in 7.5 percent Bovine Serum Albumin to keep the nanoparticles from clumping. C60 was used in concentrations ranging from 2 to 35 parts per million and incubated with bovine heart mitochondria for up to 10 minutes before measuring oxygen consumption. The rate of oxygen consumption was collected using a Clark-electrode and the data were analyzed using MS Excel. Preliminary results show that oxygen consumption from bovine heart mitochondria in the presence of NADH (Complexes I, III and IV of the electron transport chain) was affected by different concentrations of C60 or time of incubation with C60.
Faculty Mentor(s)
Carin Thomas