Glutathione as an assay for determining the oxidative ability of ultrafine particles
Document Type
Oral Presentation
Campus where you would like to present
SURC Ballroom C/D
Start Date
16-5-2013
End Date
16-5-2013
Abstract
Continued development of a quick and acellular means of measuring oxidative stress potental of ultrafine particles (UFPs) has firmly established that UFPs increase the rate at which glutathione (GSH) oxidizes in controlled solutions over time. Several GSH assays were exposed to commercially available soot particles and the rate at which concentration decayed was measured using Dithionitrobenzoic acid (Ellman's reagent) and UV visible spectroscopy. Several additional experimental control experiments such as degradation versus solution temperature and Ellman's reagent degradation due to sunlight were conducted to improve assay precision. Preliminary results have shown that a number of environmental factors such as light, solution temperature, and solution homogeneity have a profound impact on the rate of GSH oxidation. Research into the further optimization of assay parameters is being conducted to minimize the variation due to system independent variables while maintaining the practicality and expedience of the assay.
Recommended Citation
Peters, Joshua, "Glutathione as an assay for determining the oxidative ability of ultrafine particles" (2013). Symposium Of University Research and Creative Expression (SOURCE). 57.
https://digitalcommons.cwu.edu/source/2013/posters/57
Poster Number
53
Additional Mentoring Department
Environmental Studies
Glutathione as an assay for determining the oxidative ability of ultrafine particles
SURC Ballroom C/D
Continued development of a quick and acellular means of measuring oxidative stress potental of ultrafine particles (UFPs) has firmly established that UFPs increase the rate at which glutathione (GSH) oxidizes in controlled solutions over time. Several GSH assays were exposed to commercially available soot particles and the rate at which concentration decayed was measured using Dithionitrobenzoic acid (Ellman's reagent) and UV visible spectroscopy. Several additional experimental control experiments such as degradation versus solution temperature and Ellman's reagent degradation due to sunlight were conducted to improve assay precision. Preliminary results have shown that a number of environmental factors such as light, solution temperature, and solution homogeneity have a profound impact on the rate of GSH oxidation. Research into the further optimization of assay parameters is being conducted to minimize the variation due to system independent variables while maintaining the practicality and expedience of the assay.
Faculty Mentor(s)
Anne Johansen