Observing the Effects of Novel Flavonoid Malheuran-2 on MCF-7 Cells Using Flow Cytometric Analysis
Document Type
Oral Presentation
Campus where you would like to present
SURC Ballroom B/C/D
Start Date
21-5-2015
End Date
21-5-2015
Keywords
Flow Cytometry, Cancer, Cell Biology
Abstract
Apoptosis is the mechanism used to regulate the balance between cell proliferation and cell death. Reduced levels of apoptosis or increased levels of proliferation can lead to regions of uncontrollable growth, or cancer. Flavonoids are being studied because of their anti-tumor qualities and low toxicity on surrounding healthy tissue. Of six flavonoids extracted from the Dalea searlsiae plant, flavonoids 1 to 4 showed inhibitory effects on the proliferation of MCF-7 cells. After further studying the effects of flavonoid 2, apoptosis was confirmed using fluorescent microscopy and staining with Annexin V Alexa fluor 488 and propidium iodide. Analysis using flow cytometry was impossible due to false binding of Annexin which was attributed to harsh cell harvesting techniques compromising the cell membrane and surface proteins.
Recommended Citation
Hoffer, Dean, "Observing the Effects of Novel Flavonoid Malheuran-2 on MCF-7 Cells Using Flow Cytometric Analysis" (2015). Symposium Of University Research and Creative Expression (SOURCE). 35.
https://digitalcommons.cwu.edu/source/2015/posters/35
Poster Number
45
Department/Program
Biological Sciences
Additional Mentoring Department
Biological Sciences
Observing the Effects of Novel Flavonoid Malheuran-2 on MCF-7 Cells Using Flow Cytometric Analysis
SURC Ballroom B/C/D
Apoptosis is the mechanism used to regulate the balance between cell proliferation and cell death. Reduced levels of apoptosis or increased levels of proliferation can lead to regions of uncontrollable growth, or cancer. Flavonoids are being studied because of their anti-tumor qualities and low toxicity on surrounding healthy tissue. Of six flavonoids extracted from the Dalea searlsiae plant, flavonoids 1 to 4 showed inhibitory effects on the proliferation of MCF-7 cells. After further studying the effects of flavonoid 2, apoptosis was confirmed using fluorescent microscopy and staining with Annexin V Alexa fluor 488 and propidium iodide. Analysis using flow cytometry was impossible due to false binding of Annexin which was attributed to harsh cell harvesting techniques compromising the cell membrane and surface proteins.
Faculty Mentor(s)
Eric Foss