Relatedness among Camp Leakey Orangutans, Tanjung Puting, Central Kalimantan
Document Type
Oral Presentation
Campus where you would like to present
Ellensburg
Event Website
https://digitalcommons.cwu.edu/source
Start Date
15-5-2019
End Date
15-5-2019
Abstract
Genetic studies of dispersal patterns in wild populations of orangutans (Pongo spp) have sought to confirm behavioral observations that female orangutans tend to stay near their natal range while males disperse. In order to genotype a previously unsampled wild population of endangered orangutans at Tanjung Puting National Park we developed novel application of a methyl based magnetic capture for enrichment of fecal DNA and now common human targeted single nucleotide polymorphisms (SNP) microarray technology. We confirmed results of this new genotyping technique through standard microsatellite short tandem repeat (STR) micro-capillary genotyping. We estimate genetic diversity and relatedness for 32 (21 female and 9 male) wild orangutans at the Camp Leakey Study Site. We were able to successfully isolate 125 known human SNP loci (0.08% of those targeted) which hybridized orangutan DNA on the human targeted Illumina Infinium QC array. Average relatedness within the population, estimated from our SNP dataset using TrioML estimator, is at a level between half and first cousins (r= 0.0967) and we found no significant difference of r between males and females. All males and females had relatives within the study site and paternity was not assigned to any potential fathers sampled. This indicates all sampled males and females are from the local population. High and near equal relatedness for both sexes in this group combined with a low number of males sampled suggests conditions for the potential for deep inbreeding and that this population is at risk from further isolation through fragmentation.
Recommended Citation
Linsky, Ruth, "Relatedness among Camp Leakey Orangutans, Tanjung Puting, Central Kalimantan" (2019). Symposium Of University Research and Creative Expression (SOURCE). 11.
https://digitalcommons.cwu.edu/source/2019/Oralpres/11
Department/Program
Primate Behavior and Ecology
Slides for SOURCE 2019 presentation Linsky
Additional Files
RuthLinsky_SourcePresentation_May15.pptx (57702 kB)Slides for SOURCE 2019 presentation Linsky
Relatedness among Camp Leakey Orangutans, Tanjung Puting, Central Kalimantan
Ellensburg
Genetic studies of dispersal patterns in wild populations of orangutans (Pongo spp) have sought to confirm behavioral observations that female orangutans tend to stay near their natal range while males disperse. In order to genotype a previously unsampled wild population of endangered orangutans at Tanjung Puting National Park we developed novel application of a methyl based magnetic capture for enrichment of fecal DNA and now common human targeted single nucleotide polymorphisms (SNP) microarray technology. We confirmed results of this new genotyping technique through standard microsatellite short tandem repeat (STR) micro-capillary genotyping. We estimate genetic diversity and relatedness for 32 (21 female and 9 male) wild orangutans at the Camp Leakey Study Site. We were able to successfully isolate 125 known human SNP loci (0.08% of those targeted) which hybridized orangutan DNA on the human targeted Illumina Infinium QC array. Average relatedness within the population, estimated from our SNP dataset using TrioML estimator, is at a level between half and first cousins (r= 0.0967) and we found no significant difference of r between males and females. All males and females had relatives within the study site and paternity was not assigned to any potential fathers sampled. This indicates all sampled males and females are from the local population. High and near equal relatedness for both sexes in this group combined with a low number of males sampled suggests conditions for the potential for deep inbreeding and that this population is at risk from further isolation through fragmentation.
https://digitalcommons.cwu.edu/source/2019/Oralpres/11
Faculty Mentor(s)
Lori Sheeran