Title

Relatedness among Camp Leakey Orangutans, Tanjung Puting, Central Kalimantan

Document Type

Oral Presentation

Campus where you would like to present

Ellensburg

Event Website

https://digitalcommons.cwu.edu/source

Start Date

15-5-2019

End Date

15-5-2019

Abstract

Genetic studies of dispersal patterns in wild populations of orangutans (Pongo spp) have sought to confirm behavioral observations that female orangutans tend to stay near their natal range while males disperse. In order to genotype a previously unsampled wild population of endangered orangutans at Tanjung Puting National Park we developed novel application of a methyl based magnetic capture for enrichment of fecal DNA and now common human targeted single nucleotide polymorphisms (SNP) microarray technology. We confirmed results of this new genotyping technique through standard microsatellite short tandem repeat (STR) micro-capillary genotyping. We estimate genetic diversity and relatedness for 32 (21 female and 9 male) wild orangutans at the Camp Leakey Study Site. We were able to successfully isolate 125 known human SNP loci (0.08% of those targeted) which hybridized orangutan DNA on the human targeted Illumina Infinium QC array. Average relatedness within the population, estimated from our SNP dataset using TrioML estimator, is at a level between half and first cousins (r= 0.0967) and we found no significant difference of r between males and females. All males and females had relatives within the study site and paternity was not assigned to any potential fathers sampled. This indicates all sampled males and females are from the local population. High and near equal relatedness for both sexes in this group combined with a low number of males sampled suggests conditions for the potential for deep inbreeding and that this population is at risk from further isolation through fragmentation.

Faculty Mentor(s)

Lori Sheeran

Department/Program

Primate Behavior and Ecology

RuthLinsky_SourcePresentation_May15.pptx (57702 kB)
Slides for SOURCE 2019 presentation Linsky

Additional Files

RuthLinsky_SourcePresentation_May15.pptx (57702 kB)
Slides for SOURCE 2019 presentation Linsky

Share

COinS
 
May 15th, 12:00 AM May 15th, 12:00 AM

Relatedness among Camp Leakey Orangutans, Tanjung Puting, Central Kalimantan

Ellensburg

Genetic studies of dispersal patterns in wild populations of orangutans (Pongo spp) have sought to confirm behavioral observations that female orangutans tend to stay near their natal range while males disperse. In order to genotype a previously unsampled wild population of endangered orangutans at Tanjung Puting National Park we developed novel application of a methyl based magnetic capture for enrichment of fecal DNA and now common human targeted single nucleotide polymorphisms (SNP) microarray technology. We confirmed results of this new genotyping technique through standard microsatellite short tandem repeat (STR) micro-capillary genotyping. We estimate genetic diversity and relatedness for 32 (21 female and 9 male) wild orangutans at the Camp Leakey Study Site. We were able to successfully isolate 125 known human SNP loci (0.08% of those targeted) which hybridized orangutan DNA on the human targeted Illumina Infinium QC array. Average relatedness within the population, estimated from our SNP dataset using TrioML estimator, is at a level between half and first cousins (r= 0.0967) and we found no significant difference of r between males and females. All males and females had relatives within the study site and paternity was not assigned to any potential fathers sampled. This indicates all sampled males and females are from the local population. High and near equal relatedness for both sexes in this group combined with a low number of males sampled suggests conditions for the potential for deep inbreeding and that this population is at risk from further isolation through fragmentation.

https://digitalcommons.cwu.edu/source/2019/Oralpres/11