Identification of Proteins that interact with QuakingI-6
Document Type
Oral Presentation
Campus where you would like to present
Ellensburg
Event Website
https://digitalcommons.cwu.edu/source
Start Date
15-5-2019
End Date
15-5-2019
Abstract
The mammalian neocortex is the brain structure that mediates conscious thoughts and actions and is the source of human intelligence. This structure is divided into discrete areas that carry out different computational tasks. Neocortical arealization is the process through which the specific areas of the neocortex are formed, and occurs during embryonic development. In order to better understand this process, it is helpful to understand how the proteins that regulate neuronal development interact with each other. Emx2 plays an important role in controlling the size of the functional areas within the developing neocortex, the brain structure responsible for conscious thought, and interacts with each of the isoforms of a protein called QuakingI. The goal of this project is to identify proteins that interact with QuakingI-6 so to determine how it mechanistically influences neocortical arealization. To identify proteins to which QuakingI-6 interacts, a yeast two-hybrid screen was performed using QuakingI-6 as bait with a protein library. The plasmids encoding the prey proteins were rescued from the yeast cells and sequenced to identify the proteins interacting with QuakingI-6. The main proteins identified from this screen so far are β-actin, Receptor for activated kinase 1 (Rack1), and Transducin-like enhancer of split (Tle2). β-actin is a cytoskeletal protein that has many roles in the body including the developing neocortex, Rack1 is a scaffold protein that interacts with multiple cell signaling pathway, and Tle2 is a transcriptional repressor. At this point there are more assays that need to be performed to confirm these interactions.
Recommended Citation
Leon, Rosa Moreno, "Identification of Proteins that interact with QuakingI-6" (2019). Symposium Of University Research and Creative Expression (SOURCE). 82.
https://digitalcommons.cwu.edu/source/2019/Oralpres/82
Department/Program
Chemistry
Slides for SOURCE 2019 presentation Leon
Identification of Proteins that interact with QuakingI-6
Ellensburg
The mammalian neocortex is the brain structure that mediates conscious thoughts and actions and is the source of human intelligence. This structure is divided into discrete areas that carry out different computational tasks. Neocortical arealization is the process through which the specific areas of the neocortex are formed, and occurs during embryonic development. In order to better understand this process, it is helpful to understand how the proteins that regulate neuronal development interact with each other. Emx2 plays an important role in controlling the size of the functional areas within the developing neocortex, the brain structure responsible for conscious thought, and interacts with each of the isoforms of a protein called QuakingI. The goal of this project is to identify proteins that interact with QuakingI-6 so to determine how it mechanistically influences neocortical arealization. To identify proteins to which QuakingI-6 interacts, a yeast two-hybrid screen was performed using QuakingI-6 as bait with a protein library. The plasmids encoding the prey proteins were rescued from the yeast cells and sequenced to identify the proteins interacting with QuakingI-6. The main proteins identified from this screen so far are β-actin, Receptor for activated kinase 1 (Rack1), and Transducin-like enhancer of split (Tle2). β-actin is a cytoskeletal protein that has many roles in the body including the developing neocortex, Rack1 is a scaffold protein that interacts with multiple cell signaling pathway, and Tle2 is a transcriptional repressor. At this point there are more assays that need to be performed to confirm these interactions.
https://digitalcommons.cwu.edu/source/2019/Oralpres/82
Faculty Mentor(s)
Todd Kroll