Microbiome of Transgenic Non-Steroidal Anti-Inflammatory Drug Activated Gene 1 Mice
Document Type
Poster
Campus where you would like to present
Ellensburg
Event Website
https://digitalcommons.cwu.edu/source
Start Date
18-5-2020
Abstract
Recent research has shown that the composition of the gut microbiome influences obesity. A transgenic mouse model with the human non-steroidal anti-inflammatory drug (NSAID) activated gene 1 (known as NAG-1) has demonstrated an increased metabolism and obesity resistance when compared to the original strain of mice, C57BL/6, termed wild type (WT). The sequencing of the gut microbiome of the WT and the NAG-1 mice is important to further understand metabolic diseases. In this study, we are examining the microbiome of NAG-1 mice to better understand their resistance to diet-induced obesity and increased metabolism. The NAG-1 mice were compared to their WT littermates and gender differences will be examined. Preliminary comparisons were conducted to analyze metabolism and include measurements of body weight, glucose tolerance and residual organic matter in feces (a measure of energy extraction from food). The WT males were observed to have the largest change in weight and a higher mean weight than the other groups. The NAG-1 males and females were the leanest. Glucose tolerance was observed to be greatest in the NAG-1 mice. Within the combusted fecal sample data there was no significance between any of the groups, though the average remaining organic matter was consistently higher in the NAG-1 mice. At the conclusion of the experiment, caecal and colonic microbial DNA was extracted and subject to 16S rRNA sequencing. Alpha and Beta diversity analysis revealed significant differences between the microbiome of the NAG-1 and WT mice. Microbial community differences between male and female mice are significant.
Recommended Citation
Snodgrass, Chase and Reese, Safyre, "Microbiome of Transgenic Non-Steroidal Anti-Inflammatory Drug Activated Gene 1 Mice" (2020). Symposium Of University Research and Creative Expression (SOURCE). 29.
https://digitalcommons.cwu.edu/source/2020/COTS/29
Department/Program
Biological Sciences
Additional Mentoring Department
https://cwu.studentopportunitycenter.com/2020/04/microbiome-of-transgenic-non-steroidal-anti-inflammatory-drug-activated-gene-1-mice/
Microbiome of Transgenic Non-Steroidal Anti-Inflammatory Drug Activated Gene 1 Mice
Ellensburg
Recent research has shown that the composition of the gut microbiome influences obesity. A transgenic mouse model with the human non-steroidal anti-inflammatory drug (NSAID) activated gene 1 (known as NAG-1) has demonstrated an increased metabolism and obesity resistance when compared to the original strain of mice, C57BL/6, termed wild type (WT). The sequencing of the gut microbiome of the WT and the NAG-1 mice is important to further understand metabolic diseases. In this study, we are examining the microbiome of NAG-1 mice to better understand their resistance to diet-induced obesity and increased metabolism. The NAG-1 mice were compared to their WT littermates and gender differences will be examined. Preliminary comparisons were conducted to analyze metabolism and include measurements of body weight, glucose tolerance and residual organic matter in feces (a measure of energy extraction from food). The WT males were observed to have the largest change in weight and a higher mean weight than the other groups. The NAG-1 males and females were the leanest. Glucose tolerance was observed to be greatest in the NAG-1 mice. Within the combusted fecal sample data there was no significance between any of the groups, though the average remaining organic matter was consistently higher in the NAG-1 mice. At the conclusion of the experiment, caecal and colonic microbial DNA was extracted and subject to 16S rRNA sequencing. Alpha and Beta diversity analysis revealed significant differences between the microbiome of the NAG-1 and WT mice. Microbial community differences between male and female mice are significant.
https://digitalcommons.cwu.edu/source/2020/COTS/29
Faculty Mentor(s)
Holly Pinkart