Single-Cell Macrofluidics
Document Type
Oral Presentation
Campus where you would like to present
Ellensburg
Event Website
https://digitalcommons.cwu.edu/source
Start Date
18-5-2020
Abstract
Analysis of gene function in bacteria commonly involves perturbing or removing a gene product with chemical treatment and analyzing the phenotypic effects that result. Commonly this process involves using expensive and technically challenging microfluidic devices that have microscopically etched channels that deliver liquid media to individual cells. Our lab worked over Summer quarter 2019 to produce a new device called Single-Cell Macrofluidics, an inexpensive and flexible platform that still allows for fluidic control. Our main focus was to refine device design and collect data to characterize the effectiveness of delivery. We performed a variety of experiments to achieve these goals, including analyzing diffusion of fluorescent dye, estimating max rate of media exchange, and timed diffusion of media to live cells. This was a preliminary study with promising results, but more work is needed to refine our delivery system and overall structure of the fluidic device.
Recommended Citation
Heath, Mason, "Single-Cell Macrofluidics" (2020). Symposium Of University Research and Creative Expression (SOURCE). 82.
https://digitalcommons.cwu.edu/source/2020/COTS/82
Department/Program
Physics
Additional Mentoring Department
https://cwu.studentopportunitycenter.com/2020/04/single-cell-macrofluidics/
Single-Cell Macrofluidics
Ellensburg
Analysis of gene function in bacteria commonly involves perturbing or removing a gene product with chemical treatment and analyzing the phenotypic effects that result. Commonly this process involves using expensive and technically challenging microfluidic devices that have microscopically etched channels that deliver liquid media to individual cells. Our lab worked over Summer quarter 2019 to produce a new device called Single-Cell Macrofluidics, an inexpensive and flexible platform that still allows for fluidic control. Our main focus was to refine device design and collect data to characterize the effectiveness of delivery. We performed a variety of experiments to achieve these goals, including analyzing diffusion of fluorescent dye, estimating max rate of media exchange, and timed diffusion of media to live cells. This was a preliminary study with promising results, but more work is needed to refine our delivery system and overall structure of the fluidic device.
https://digitalcommons.cwu.edu/source/2020/COTS/82
Faculty Mentor(s)
Nathan Kuwada