Title

Single-Cell Macrofluidics

Document Type

Oral Presentation

Campus where you would like to present

Ellensburg

Event Website

https://digitalcommons.cwu.edu/source

Start Date

18-5-2020

Abstract

Analysis of gene function in bacteria commonly involves perturbing or removing a gene product with chemical treatment and analyzing the phenotypic effects that result. Commonly this process involves using expensive and technically challenging microfluidic devices that have microscopically etched channels that deliver liquid media to individual cells. Our lab worked over Summer quarter 2019 to produce a new device called Single-Cell Macrofluidics, an inexpensive and flexible platform that still allows for fluidic control. Our main focus was to refine device design and collect data to characterize the effectiveness of delivery. We performed a variety of experiments to achieve these goals, including analyzing diffusion of fluorescent dye, estimating max rate of media exchange, and timed diffusion of media to live cells. This was a preliminary study with promising results, but more work is needed to refine our delivery system and overall structure of the fluidic device.

Faculty Mentor(s)

Nathan Kuwada

Department/Program

Physics

Additional Mentoring Department

https://cwu.studentopportunitycenter.com/2020/04/single-cell-macrofluidics/

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COinS
 
May 18th, 12:00 PM

Single-Cell Macrofluidics

Ellensburg

Analysis of gene function in bacteria commonly involves perturbing or removing a gene product with chemical treatment and analyzing the phenotypic effects that result. Commonly this process involves using expensive and technically challenging microfluidic devices that have microscopically etched channels that deliver liquid media to individual cells. Our lab worked over Summer quarter 2019 to produce a new device called Single-Cell Macrofluidics, an inexpensive and flexible platform that still allows for fluidic control. Our main focus was to refine device design and collect data to characterize the effectiveness of delivery. We performed a variety of experiments to achieve these goals, including analyzing diffusion of fluorescent dye, estimating max rate of media exchange, and timed diffusion of media to live cells. This was a preliminary study with promising results, but more work is needed to refine our delivery system and overall structure of the fluidic device.

https://digitalcommons.cwu.edu/source/2020/COTS/82