Inoculation of Mice against Leishmania major by Immunization with Purified Paraflagellar Rod Proteins
Document Type
Oral Presentation
Campus where you would like to present
SURC Ballroom A
Start Date
17-5-2012
End Date
17-5-2012
Abstract
Leishmania major is a single-celled, vector-borne parasite responsible for the disease cutaneous leishmaniasis. This infection leads to disfiguring skin ulcers at the bite-site, which self-heal in immune competent individuals. Leishmaniasis has become an emerging zoonotic disease not only in the United States but worldwide. This can be attributed to increased global travel for military and recreational purposes and the disruption of vector habitats through industrialization in endemic areas. The World Health Organization estimates that Leishmania infects some 12 million people in 88 countries with an estimated 2 million new cases every year. Studies in a related parasite, Trypanosoma cruzi, have established that the paraflagellar rod (PFR) proteins of the flagellum are highly immunogenic and can protect mice from succumbing to infection. The PFR is a unique protein lattice that runs along the flagellum found only in the order kinetoplastids, to which both Leishmania and Trypanosoma belong. The aim of our research is to investigate the immune potential of the PFR proteins derived from L. major. This study used a refined protocol of protein purification of the PFR to test the protective immune response generated in mice. In this trial, PFR and Freund's adjuvant were combined to immunize highly susceptible BALB/c mice, compared to a control mice immunized with Freund’s adjuvant alone. The lesion size in the immunized and control mice was followed over a 5 month period. Data from this study will be used to design further trials to refine and improve the immunization protocol.
Recommended Citation
Anderson, Heidi and Reis, Ashleigh, "Inoculation of Mice against Leishmania major by Immunization with Purified Paraflagellar Rod Proteins" (2012). Symposium Of University Research and Creative Expression (SOURCE). 7.
https://digitalcommons.cwu.edu/source/2012/posters/7
Poster Number
15
Additional Mentoring Department
Biological Sciences
Inoculation of Mice against Leishmania major by Immunization with Purified Paraflagellar Rod Proteins
SURC Ballroom A
Leishmania major is a single-celled, vector-borne parasite responsible for the disease cutaneous leishmaniasis. This infection leads to disfiguring skin ulcers at the bite-site, which self-heal in immune competent individuals. Leishmaniasis has become an emerging zoonotic disease not only in the United States but worldwide. This can be attributed to increased global travel for military and recreational purposes and the disruption of vector habitats through industrialization in endemic areas. The World Health Organization estimates that Leishmania infects some 12 million people in 88 countries with an estimated 2 million new cases every year. Studies in a related parasite, Trypanosoma cruzi, have established that the paraflagellar rod (PFR) proteins of the flagellum are highly immunogenic and can protect mice from succumbing to infection. The PFR is a unique protein lattice that runs along the flagellum found only in the order kinetoplastids, to which both Leishmania and Trypanosoma belong. The aim of our research is to investigate the immune potential of the PFR proteins derived from L. major. This study used a refined protocol of protein purification of the PFR to test the protective immune response generated in mice. In this trial, PFR and Freund's adjuvant were combined to immunize highly susceptible BALB/c mice, compared to a control mice immunized with Freund’s adjuvant alone. The lesion size in the immunized and control mice was followed over a 5 month period. Data from this study will be used to design further trials to refine and improve the immunization protocol.
Faculty Mentor(s)
Gabrielle Stryker, Blaise Dondji